Fig 1: Effect of old age on microbiota composition and intestinal barrier in male mice. Principal Coordinate Analysis (PCoA) depicting the microbial communities of the samples from (A) small and (B) large intestine (each dot represents one sample) and area plot showing the abundances of Gram-positive and Gram-negative of the classified genera (samples are separated by age) of (C) small and (D) large intestine (x-axis showing individual samples). Representative pictures of hematoxylin and eosin stained (E) small intestine and (F) large intestine (200×), (G) xylose permeation (unpaired t-test: small intestine: p < 0.05, large intestine: ns p > 0.05) in everted gut sacs, densitometric analysis of (H) occludin (small intestine: Mann–Whitney-test: p < 0.01, large intestine: unpaired t-test: ns p > 0.05) and (I) zonula occludens-1 (ZO-1) (unpaired t-test: small intestine: p < 0.001, large intestine: ns p > 0.05) in immunohistochemical stained (IHC staining) small and large intestinal sections. (J) Intestinal-fatty acid binding protein (I-FABP) concentration (unpaired t-test: p < 0.01) in plasma and (K) cluster of differentiation 14 (CD14) protein concentration (unpaired t-test: p < 0.01) in Western blots of plasma, as well as ligands of Toll-like receptor 2 (TLR2) and TLR4 in (L) plasma from portal vein (v.p.) (unpaired t-test: TLR2 ligands: p < 0.001, TLR4 ligands: p < 0.001) and (M) retrobulbar plasma (unpaired t-test: TLR2 ligands: p < 0.05, TLR4 ligands: p < 0.05) of 4-month-old (mo) and 24-month-old mice. Data are presented as means ± SEM, n = 6–8, see Section for further details, * p < 0.05, ns = not significant.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Mouse FABP2/I-FABP ELISA Kit (Colorimetric)